Abstract
Freezing of chemically untreated mucoid-containing salivary secretions for varied periods of time has been observed to result in the formation of a persisting coagulum when the secretion is thawed. The coagulum, or clot, generally resembles in appearance the mucin clot formed in response to the addition of acid. Evidence from viscosity measurements, response to dilute acetic acid, and analyses of sialic acid indicates that the coagulum formed after freezing and thawing is derived from the salivary mucoid.
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