The isolation of bradykinin, a plasma kinin from ox blood
- 1 January 1961
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 78 (1) , 60-65
- https://doi.org/10.1042/bj0780060
Abstract
Bradykinin was formed by trypsin digestion of the fraction of ox serum proteins which precipitated between 33 and 45% of saturation with ammonium sulphate. Bradykinin was isolated in the pure state by application of the techniques of countercurrent distribution, chromato-graphy on carboxymethylcellulose and paper electrophoresis. Bradykinin contained the five amino acids glycine, serine, proline, phenyl-alanine and arginine in the molar proportions 11:3:2:2 and one of the arginine residues was N-terminal.Keywords
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