Structure of Mitochondria and Vacuoles of Candida utilis and Schizosaccharomyces pombe Studied by Electron Microscopy of Serial Thin Sections and Model Building

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Abstract
The structure of mitochondria and of vacuoles in C. utilis and S. pombe was studied by EM of serial thin sections and subsequent model building. The models of the 2 cells of C. utilis which were studied confirmed our earlier findings, made by high voltage EM of thick sections, that there is a single, branched and continuous mitochondrial network in the cell. A model of a S. pombe cell showed that the mitochondrial structure was far more continuous than expected from inspection of thin sections, there being but 2 large and 2 small mitochondria. The models demonstrated that the few large vacuoles in C. utilis were interconnected into a single cluster, whereas in S. pombe there were 2 separate complexes of interconnected vacuoles towards each pole of the cell.

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