DNA-REPAIR SYNTHESIS FOLLOWING EXPOSURE TO CHEMICAL MUTAGENS IN PRIMARY LIVER, STOMACH, AND INTESTINAL-CELLS ISOLATED FROM RAINBOW-TROUT

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  • 1 January 1984
    • journal article
    • research article
    • Vol. 44  (3) , 1120-1121
Abstract
DNA repair synthesis was autoradiographically measured in liver, stomach, and intestinal cells isolated from rainbow trout which were exposed in vitro to the chemical mutagens, N-methyl-N''-nitro-N-nitrosoguanidine, 4-nitroquinoline 1-oxide, and aflatoxin B1. The level of repair was greatest in primary hepatocytes which responded to all three mutagens. Only nominal amounts of repair were detected in stomach cells following N-methyl-N''-nitro-N-nitrosoguanidine and 4-nitroquinoline 1-oxide exposures and in intestinal cells following 4-nitroquinoline 1-oxide exposure. In comparison with cultured rainbow trout cells, the quantity of DNA repair found in primary cells is significantly less. [The implications for the use of rainbow trout in carcinogen assays are discussed.].