Uteroferrin has N-asparagine-linked high-mannose-type oligosaccharides that contain mannose 6-phosphate.
- 1 May 1984
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 81 (10) , 2985-2989
- https://doi.org/10.1073/pnas.81.10.2985
Abstract
Uteroferrin is an iron-containing, progesterone-induced, acid phosphatase that is secreted in large amounts by the uterine endometrium of pigs. During pregnancy, it transports iron across the chorioallantois (placenta) for use in fetal hematopoiesis. In this paper, it is reported that uteroferrin synthesized by cultured endometrial explants possesses N-linked, high-mannose, oligosaccharide chains that contain 6- phosphomannose units. The latter is regarded as a possible recognition marker whereby acid hydrolases are targeted to the lysosome. On uteroferrin, however, the majority of the phosphate is in single diester linkages between the mannose and a covering N-acetylglucosamine. It is suggested that uteroferrin is a lysosomal enzyme that has assumed a role in iron transport and metabolism and is secreted because the covering N-acetylglucosamine is not removed.This publication has 39 references indexed in Scilit:
- The Cytochemistry of Tartrate-resistant Acid Phosphatase: Technical ConsiderationsAmerican Journal of Clinical Pathology, 1978
- Iron-containing acid phosphatases: Comparison of the enzymes from beef spleen and pig allantoic fluidBiochemical and Biophysical Research Communications, 1978
- High resolution two-dimensional electrophoresis of basic as well as acidic proteinsCell, 1977
- A study of prostaglandin F2α as the luteolysin in swine: III effects of estradiol valerate on prostaglandin F, progestins, estrone and estradiol concentrations in the utero-ovarian vein of nonpregnant giltsProstaglandins, 1977
- Biochemical characterization of the tartrate-resistant acid phosphatase of human spleen with leukemic reticuloendotheliosis as a pyrophosphatase.Clinical Chemistry, 1977
- Requirement of an essential thiol group and ferric iron for the activity of the progesterone-induced porcine uterine purple phosphatase.Journal of Biological Chemistry, 1976
- An Iron-containing Phosphatase Induced by Progesterone in the Uterine Fluids of PigsJournal of Biological Chemistry, 1974
- Purification and Properties of a Progesterone-induced Basic Glycoprotein from the Uterine Fluids of PigsJournal of Biological Chemistry, 1973
- Selective enzyme purification by affinity chromatography.Proceedings of the National Academy of Sciences, 1968
- The Intracellular Location of Phosphoprotein Phosphatase ActivityJournal of Biological Chemistry, 1959