Amino Acid Incorporation into Liver Proteins during Extrahepatic Cholestasis in the Rat

Publisher Website
Google Scholar
Abstract
Extrahepatic cholestasis was induced in rats by ligation of the bile duct. L-(4,5-3H)-leucine or L-(U-14C)-leucine were incorporated into the proteins of control and cholestatic liver slices by incubation in balanced salt media. The cholestatic liver showed a rate of amino acid incorporation which was approximately three times that of control liver. There was no apparent effect on the specific radioactivity of the precursor. The distribution of protein-bound radioactivity among sub-cellular fractions was studied. The radioactivity increased in all the main fractions, and the largest increase was seen in the soluble proteins. Serum from control or cholestatic animals and bile from control animals added during incubation of liver slices had small effect on the incorporation of leucine. Leucine incorporation was not appreciably altered in other organs.

This publication has 0 references indexed in Scilit: