Effects of Detergents, Proteins, and Lipids on 2′:3′‐Cyclic‐Nucleotide 3′‐Phosphodiesterase Activity
- 1 July 1980
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 35 (1) , 164-169
- https://doi.org/10.1111/j.1471-4159.1980.tb12502.x
Abstract
The myelin marker 2′:3′‐cyclic‐nucleotide 3′‐phosphodiesterase (CNPase) was isolated to a lipid‐ and phosphate‐free stage. The effects of exogenously added lipids were tested on this preparation and compared to the known stimulation of the enzyme by detergents and proteins. CNPase could be stimulated 2–3 fold by these various agents which appeared to be additive in their effect. Enzyme‐protein and enzyme‐lipid interactions and possible medical use of the improved assay conditions for CNPase employed in the study are discussed.Keywords
This publication has 23 references indexed in Scilit:
- Activity of 2′,3′‐cyclic nucleotide 3′‐phosphohydrolase in human cerebrospinal fluidAnnals of Neurology, 1979
- PURIFICATION AND COMPARISON OF 2′,3′‐CYCLIC NUCLEOTIDE 3′‐PHOSPHOHYDROLASES FROM BOVINE BRAIN AND SPINAL CORDJournal of Neurochemistry, 1978
- LOCALIZATION OF 2′,3′‐CYCLIC NUCLEOTIDE‐3′‐PHOSPHOHYDROLASE OF RABBIT BRAIN BY SEDIMENTATION IN A CONTINUOUS SUCROSE GRADIENT1Journal of Neurochemistry, 1978
- ACTIVITY OF 2′,3′‐CYCLIC‐NUCLEOTIDE 3′‐PHOSPHODIESTERASE IN REGIONS OF RAT BRAIN DURING DEVELOPMENT: QUANTITATIVE RELATIONSHIP TO MYELIN BASIC PROTEINJournal of Neurochemistry, 1978
- EFFECT OF EXOGENOUS LIPIDS ON ACTIVITIES OF THE RAT BRAIN CHOLESTEROL ESTER HYDROLASE LOCALIZED IN THE MYELIN SHEATHJournal of Neurochemistry, 1976
- The role of phospholipid acyl chains in the activation of mitochondrial ATPase complexBiochimica et Biophysica Acta (BBA) - Biomembranes, 1975
- Effect of exogenous lipids on rat brain cholesterol ester hydrolasesExperimental Neurology, 1974
- The Molecular Organization of MembranesAnnual Review of Biochemistry, 1974
- Membrane-bound enzymes and membrane ultrastructureBiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1973
- THE REGIONAL AND SUBCELLULAR DISTRIBUTION OF 2′,3′‐CYCLIC NUCLEOTIDE 3′‐PHOSPHOHYDROLASE IN THE CENTRAL NERVOUS SYSTEMJournal of Neurochemistry, 1967