Pigment Composition and Optical Rotatory Dispersion of Chloroplast Fractions Obtained by Detergent Action
- 1 June 1966
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 41 (6) , 1081-1082
- https://doi.org/10.1104/pp.41.6.1081
Abstract
Spinach chloroplasts after digitonin or Triton treatment and differential centrifugation yielded a light and heavy fraction. The light and heavy fractions obtained by treating with different detergents had a remarkably constant pigment distribution. The heavy fractions had a chlorophyll a/b ratio of 2; that of the light fractions, between 6 and 7. The light fractions contained 2-3 times more [beta]-carotene than the heavy fractions. The heavy fractions contained more xanthophylls, with lutein being the major pigment besides the chlorophylls. The chlorophyll distribution in the 2 fractions could be discerned from the absorption and ORD spectra. The large Cotton effect with a negative trough near 478 m[mu] probably originated from either lutein or chlorophyll b.This publication has 2 references indexed in Scilit:
- Two pigment proteins in spinach chloroplastsBiochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis, 1966
- Optical rotatory dispersion of chloroplast-lamellae fragmentsArchives of Biochemistry and Biophysics, 1965