High-Throughput SNP Genotyping by Single-Tube PCR with T m -Shift Primers
Open Access
- 1 December 2005
- journal article
- Published by Taylor & Francis in BioTechniques
- Vol. 39 (6) , 885-893
- https://doi.org/10.2144/000112028
Abstract
Despite many recent advances in high-throughput single nucleotide polymorphism (SNP) genotyping technologies, there is still a great need for inexpensive and flexible methods with a reasonable thro... Curve Call Just as batters know curve balls are hard to hit, genotypers are all too aware that calling SNPs from real-time PCR melting curves can prove fiendishly difficult. The general strategy wo...Keywords
Funding Information
- Brian Rhees, Mitchell Martin, Jia Li, and Henry Erlich
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