An Automated HPLC Assay for Simultaneous Quantitation of Methylated Xanthines and Uric Acids in Urine
- 1 April 1982
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Chromatographic Science
- Vol. 20 (4) , 155-159
- https://doi.org/10.1093/chromsci/20.4.155
Abstract
To investigate the elimination kinetics of caffeine and its metabolites, as well as the interaction between them, an automated HPLC method is described. This method involves a single extraction procedure, followed by a gradient elution. Fourteen methylated xanthines and uric acids are well separated with an assay sensitivity of 1 μg/ml when one-half ml of urine is used. The assay is highly selective, from endogenous compounds, and reproducible. This method is recommended for accurate pharmacokinetic studies.Keywords
This publication has 0 references indexed in Scilit: