Detection of Sulfa Drugs and Antibiotics in Milk
Open Access
- 1 January 1971
- journal article
- research article
- Published by American Society for Microbiology in Applied Microbiology
- Vol. 21 (5) , 806-808
- https://doi.org/10.1128/aem.21.5.806-808.1971
Abstract
A disc assay method for testing sulfa drugs and antibiotics in milk was developed wherein Bacillus megaterium ATCC 9855 was used as the test organism and Mueller-Hinton agar was used as the test substrate. Incubation was at 37 C for 4 to 5 hr. The test procedure is an improvement over the Bacillus subtilis-Antibiotic Medium No. 1 method, as described in Standard Methods for the Examination of Dairy Products, in that it is sensitive to eight sulfa drugs and to bacitracin without a significant change in sensitivity to eight other antibiotics commonly used for mastitis therapy.Keywords
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