Use of an Escherichia coli beta-glucuronidase gene as a reporter gene for investigation of Bacteroides promoters
- 1 July 1991
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 173 (14) , 4540-4543
- https://doi.org/10.1128/jb.173.14.4540-4543.1991
Abstract
We have constructed transcriptional fusion vectors for use in Bacteroides spp., a genus of gram-negative obligate anaerobes found in high numbers in the human colon. The reporter group in these vectors is a promoterless beta-glucuronidase gene from Escherichia coli (uidA). Two of the vectors (pMJF-2 and pMJF-3) replicate in Bacteroides spp. The third, pCQW-1, does not replicate in Bacteroides spp. and can be used to introduce E. coli beta-glucuroindase fusions into the Bacteroides chromosome.Keywords
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