Aequorea green fluorescent protein
- 21 March 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 341 (2-3) , 277-280
- https://doi.org/10.1016/0014-5793(94)80472-9
Abstract
Expression of the cDNA for Aequorea green fluorescent protein in E. coli yielded a fused protein with fluorescence excitation and emission spectra virtually identical to those of the native green fluorescent protein. Further, a solution of the protein, when mixed with aequorin and calcium ion, emitted a greenish luminescence characteristic of the in vivo luminescence of the animal, indicating a radiationless energy transfer to the protein.Keywords
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