EFFECT OF L-PHENYLALANINE MUSTARD, ADRIAMYCIN, ACTINOMYCIN-D, AND 4'-(9-ACRIDINYLAMINO)METHANESULFON-META-ANISIDIDE ON NATURALLY-OCCURRING HUMAN SPONTANEOUS MONOCYTE-MEDIATED CYTO-TOXICITY

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  • 1 January 1982
    • journal article
    • research article
    • Vol. 42  (5) , 1692-1695
Abstract
Cis-diamminedichloroplatinum(II) (cis-DDP), an active antineoplastic agent, and X-irradiation enhanced human spontaneous monocyte-mediated cytotoxicity in vitro. To ascertain whether this is a unique property of cis-DDP or common to other effective antineoplastic agents, the effects of L-phenylalanine mustard (L-PAM), adriamycin (ADR), actinomycin D and 4''-(9-acridinylamino)methanesulfon-m-anisidide (m-AMSA) on spontaneous monocyte-mediated cytotoxicity were studied. Cis-DDP, ADR, L-PAM and m-AMSA under appropriate in vitro conditions all increased spontaneous monocyte-mediated cytotoxicity. Activation involved direct monocyte activation (cis-DDP and ADR); inactivation of suppressor lymphocytes (X-irradiation); a combination of the 2 (L-PAM); or mechanisms not yet elucidated (m-AMSA). Incubating mononuclear leukocytes with 0.4 .mu.g actinomycin D/ml depressed monocyte-mediated killing. Thus, while stimulation of nonspecific killing is not a unique property of cis-DDP, it is not a universal effect of all chemotherapeutic agents. The stimulation and enhancement of already existing host defense mechanisms may be an important additional way in which many chemotherapeutic agents exert their antitumor effect.