Some Effects of Inorganic Salts on the Blood Specific Gravity and Tissue Fluids of the Bluegill Lepomis macrochirus Raf.

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Abstract
The changes in the blood sp. gr. and in the % tissue fluids of the bluegill, L. macrochirus, were detd. for fish subjected to the following solns. (concns. in ppm.) Na2SO4 17,500; NaCl 14,500; CaCl2 8400; Na2HPO4 5800; KC1 5500; Na2CrO4 930; Na2Cr2O7 728; Na2CO3 387. The blood detns. were made using the xylene-bromobenzene falling-drop densiometer. Blood was collected by puncture of the bulbus arteriosus with a hypodermic needle-tube. The percent tissue fluids in the musculature was detd. by drying the tissue to constant wt. (110[degree]C). The technics are descr. The solns. of CaCl2, KC1, and NaCl caused decreases in the percent tissue fluids; increases were caused by the solns. of Na2CO3 and Na2Cr2O7- The blood sp. gr values of fish subjected to the solns. of NaCl and Na2Cr2O7 were lower than their respective control group values. A special series of tests using phenol red (1:10,000) showed that L. macrochirus ingests water in normal as well as hypertonic environments. The tests also showed that the mucous covering of the body was altered by the salts used. It is postulated that the tissue changes reported above might here been effected through the body surface and the epithelium of the digestive tract as well as through the gills and oral membranes.
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