Formation of Free Fatty Acid and Ceramide During Brain Handling: Lability of Sphingomyelin
- 1 April 1985
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 44 (4) , 1152-1155
- https://doi.org/10.1111/j.1471-4159.1985.tb08737.x
Abstract
Intact brain and brain homogenates readily form free fatty acids and ceramides, even in the cold during subcellular isolation procedures. The fatty acid formation is slightly stimulated by chelators and might be due to phospholipid hydrolysis by lysosomal phospholipases. The ceramide formation is accompanied by loss of sphingomyelin and is apparently due to the action of neutral, metal ion-activated sphingomyelinase. The latter reaction is inhibited by EDTA whereas both degradative processes are inhibited by mercuriphenylsulfonate, the thiol-reacting inhibitor. Cerebroside does not seem to be a source of accumulated ceramide.Keywords
This publication has 18 references indexed in Scilit:
- Stimulation of Free Fatty Acid and Diacylglycerol Accumulation in Cerebrum and Cerebellum During Bicuculline‐Induced Status Epilepticus. Effect of Pretreatment with α‐Methyl‐p‐Tyrosine and p‐ChlorophenylalanineJournal of Neurochemistry, 1983
- Determination of glucocerebroside, sphingomyelin, free fatty acid and total lipid by thin-layer chromatography and charring-scintillation quenchingBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1982
- Quantitation of lipids by charring on thin-layer plates and scintillation quenching: Application to ceramide determinationAnalytical Biochemistry, 1981
- The binding of 18S acetylcholinesterase to sphingomyelin and the role of the collagen-like tailBiochemical and Biophysical Research Communications, 1977
- Ceramidase and ceramide synthesis in human kidney and cerebellumBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1975
- Brain free fatty acid levels in rats sacrificed by decapitation versus focused microwave irradiationLipids, 1975
- The inhibitor-sensitive sites of galactosyl ceramide galactosidaseArchives of Biochemistry and Biophysics, 1973
- Effects of ischemia and electroconvulsive shock on free fatty acid pool in the brainBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1970
- The production of unesterified fatty acid in brainBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1968