The influence of the purine 2-amino group on DNA conformation and stability. Synthesis and conformational analysis of d[T(2-aminoA)]3
- 1 January 1982
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 10 (14) , 4351-4361
- https://doi.org/10.1093/nar/10.14.4351
Abstract
A self-complementary hexanucleotide consisting of thymidine and 2-amino-deoxyadenosine, d(TA')3, has been synthesized by a solid phase phosphotriester method. Melting studies show that the additional hydrogen bond afforded by the 2-amino group substantially stabilizes the duplex. Moreover, conformational analysis using circular dichroism shows that a salt-induced conformational transition occurs, similar to the B leads to Z transition observed for d(CG)n oligonucleotides.Keywords
This publication has 29 references indexed in Scilit:
- DNA and RNA oligomer thermodynamics: The effect of mismatched bases on double‐helix stabilityBiopolymers, 1981
- Changes in the helical repeat of poly(dG-m5 dC) · poly(dG-m5dC) and poly(dG-dC) · poly(dG-dC) associated with the B—Z transitionNature, 1981
- Structure of a B-DNA dodecamerJournal of Molecular Biology, 1981
- Left-handed DNA in restriction fragments and a recombinant plasmidNature, 1981
- Thermodynamics of (dG-dC)3 double-helix formation in water and deuterium oxideBiochemistry, 1981
- Effect of tetramethylammonium ion on the helix-to-coil transition of poly(deoxyadenylylthymidine): a nuclear magnetic resonance and calorimetric investigationBiochemistry, 1981
- Left-Handed Double Helical DNA: Variations in the Backbone ConformationScience, 1981
- A compact form of double‐stranded DNA in solutionFEBS Letters, 1972
- Salt-induced co-operative conformational change of a synthetic DNA: Equilibrium and kinetic studies with poly(dG-dC)Journal of Molecular Biology, 1972
- Oligonucleotide interactions. III. Circular dichroism studies of the conformation of deoxyoligonucleolidesBiopolymers, 1970