Surface-Plasmon-Mediated Single-Molecule Fluorescence Through a Thin Metallic Film
- 19 January 2005
- journal article
- research article
- Published by American Physical Society (APS) in Physical Review Letters
- Vol. 94 (2) , 023005
- https://doi.org/10.1103/physrevlett.94.023005
Abstract
We demonstrate that fluorescence of single molecules in the nanometric vicinity of a thin gold film can be effectively excited and detected through the film with an epi-illumination scanning confocal microscope. A full theoretical treatment of the fluorescence signal indicates that both excitation and emission are surface-plasmon mediated. Remarkably, the number of photons detectable from chromophores perpendicular to the interface is enhanced by the presence of the metal.Keywords
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