Comparative study of rye and thymus histones: amino acid analysis and tryptic fingerprinting

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Abstract
Amino acid compositiond tryptic fingerprints of rye (Secale cereale) H1, H2B (PHI), and H2A (PHII) histones indicate major differences between these and the corresponding calf or rabbit fractions. Besides variations for other amino acids, fraction H1 from rye contains twice as much arginine as the corresponding animal fraction; the plant H2B (PHI) and H2A (PHII) histones show lysine to arginine ratios greater than those of their animal counterparts. Tryptic maps of the same proteins appear to differ between plants and animals by the number and general pattern of the peptides and by quantity and distribution of the arginine-containing peptides. There may be differences in the primary structure of the calf and rye lysine-rich and moderately lysine-rich histones. Apparently each of these plant histones cannot consist of an animal-like protein with an additional segment of 20-30 amino acid residues. The rye and calf arginine-rich fractions H3 and H4 show similar amino acid compositions and tryptic peptides maps.

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