Chromatography of histones

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Abstract
A description is given of a chromatographic system capable of separating the histones of calf thymus and other tissues into the lysine-rich histone and the remainder, with a recovery higher than 90%. Analysis shows that the lysine: arginine ratio decreases in the histone eluted with higher concentrations of salt, but it could not be decided whether the histones consisted of a few limited species or were more complex. Examination of the protein in the acid extracts of nuclear materials from calf thymus and other tissues has demonstrated the presence of non-basic protein contaminants.