Dimethyl Sulfoxide and Related Compounds. Some Effects on Human Fibroblasts in vitro.

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Abstract
When human fibroblasts were cultured in medium containing 1% DMSO, [dimethylsulfoxide] a decrease in mitotic rate and an increase in cell hydroxyproline concentration was noted when compared with control cultures grown in the same medium but without DMSO. A constant one generation time lag behind control cultures was noted in growth experiments by 48-72 hr. and a 3 fold increase in cell hydroxyproline concen-trationwas notedin the DMSO treated cultures after varying periods of time. Additional experiments were carried out in identical fashion using 1% acetone (a DMSO analogue) and 1% methanol with a resulting 4-5 fold increase in cell hydroxyproline concentration for acetone treated cultures and a 2 fold increase for the methanol treated cultures when compared to matched control cultures.