Gene Discovery in Boophilus microplus, the Cattle Tick: The Transcriptomes of Ovaries, Salivary Glands, and Hemocytes
- 1 October 2004
- journal article
- research article
- Published by Wiley in Annals of the New York Academy of Sciences
- Vol. 1026 (1) , 242-246
- https://doi.org/10.1196/annals.1307.037
Abstract
Abstract: The quest for new control strategies for ticks can profit from high throughput genomics. In order to identify genes that are involved in oogenesis and development, in defense, and in hematophagy, the transcriptomes of ovaries, hemocytes, and salivary glands from rapidly ingurgitating females, and of salivary glands from males of Boophilus microplus were PCR amplified, and the expressed sequence tags (EST) of random clones were mass sequenced. So far, more than 1,344 EST have been generated for these tissues, with approximately 30% novelty, depending on the the tissue studied. To date approximately 760 nucleotide sequences from B. microplus are deposited in the NCBI database. Mass sequencing of partial cDNAs of parasite genes can build up this scant database and rapidly generate a large quantity of useful information about potential targets for immunobiological or chemical control.Keywords
This publication has 3 references indexed in Scilit:
- High-throughput approaches to study salivary proteins and genes from vectors of diseaseInsect Biochemistry and Molecular Biology, 2002
- Toward a description of the sialome of the adult female mosquito Aedes aegyptiInsect Biochemistry and Molecular Biology, 2002
- Insecticides and acaricides : resistance and environmental impactRevue Scientifique et Technique de l'OIE, 1994