A model for tumor suppression using H-1 parvovirus.
- 15 September 1993
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 90 (18) , 8702-8706
- https://doi.org/10.1073/pnas.90.18.8702
Abstract
A model system is proposed to investigate, at the molecular level, the pathways of tumor suppression. As a tool for the selection of cells with a suppressed phenotype, we used the H-1 parvovirus that preferentially kills various neoplastic cells. From the human K562 leukemia cells, we isolated a clone, KS, that is resistant to the cytopathic effect of the H-1 virus and displays a suppressed malignant phenotype. The suppressed malignancy and the cellular resistance to H-1 killing appear to depend on the activity of wild-type p53. Whereas the KS cells express wild-type p53, the protein is undetectable in the parental K562 cells. Experiments with p53 mutants suggest that wild-type p53, in its functionally intact state, contributes to the resistance against the cytopathic effect of H-1 parvovirus.Keywords
This publication has 20 references indexed in Scilit:
- Antineoplasic activity of parvovirusesJournal of Virological Methods, 1991
- Detection of genes with a potential for suppressing the transformed phenotype associated with activated ras genes.Proceedings of the National Academy of Sciences, 1989
- Suppression of the Neoplastic Phenotype by Replacement of the RB Gene in Human Cancer CellsScience, 1988
- Revertants of v-fos-transformed fibroblasts have mutations in cellular genes essential for transformation by other oncogenesCell, 1987
- Introduction of a Normal Human Chromosome 11 into a Wilms' Tumor Cell Line Controls Its Tumorigenic ExpressionScience, 1987
- A Sequence in M13 Phage Detects Hypervariable Minisatellites in Human and Animal DNAScience, 1987
- The Autonomously Replicating Parvoviruses of VertebratesPublished by Elsevier ,1987
- A human DNA segment with properties of the gene that predisposes to retinoblastoma and osteosarcomaNature, 1986
- Flat revertants isolated from Kirsten sarcoma virus-transformed cells are resistant to the action of specific oncogenes.Proceedings of the National Academy of Sciences, 1983
- Suppression of Malignancy by Cell FusionNature, 1969