Abstract
Chromosomal analysis of 1st-cleavage mouse embryos obtained by fertilization of TO eggs with TO spermatozoa in vitro revealed that the incidence of polyploidy was proportional to the dose of PMSG [pregnant mare''s serum gonadotropin] given to the donor females. The dose-response was linear, with the level of polyploidy rising from 8.0% with a PMSG dose of 1.5 IU/female to 20.8% with 10.0 IU/female. A total of 64 polyploid embryos were found among the 452 examined and in all cases the polyploidy was the result of polyspermy or fertilization by diploid spermatozoa.

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