Tritium sequence analysis of oligoribonucleotides: a combination of post-labeling and thin-layer chromatographic techniques for the analysis of partial snake venom phosphodiesterase digests
- 1 January 1974
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 1 (10) , 1329-1342
- https://doi.org/10.1093/nar/1.10.1329
Abstract
A tritium derivative method for sequence analysis of polyribonucleotides is detailed, which is based on borotritide reduction of oligonucleotide-3' dialdehydes generated by controlled snake venom phosphodiesterase/alkaline phosphomonoesterase digestion and periodate treatment of time point aliquots of the incubation mixture. Radioactive oligonucleotide derivatives are resolved according to chain length by PEI-cellulose(1) anion-exchange TLC and their 3'-termini identified by techniques described in the preceding paper of this series(2). The present tritium derivative method is compared with the one described previously(2).Keywords
This publication has 19 references indexed in Scilit:
- DNA Sequencing TechniquesAnnual Review of Biochemistry, 1974
- Tritium sequence analysis of polyribonucleotides following periodate‐phosphomonoesterase degradation — characterization of oligonucleotide‐3′ dialdehyde intermediatesFEBS Letters, 1974
- Derivatives ofN-[N-(9-β-D-ribofuranosylpurin-6-yl)carbamoyl]threonine in phosphodiesterase hydrolysates of wheat embryo transfer ribonucleic acidBiochemistry, 1974
- Continuous directional degradation — a novel method for sequence analysis of polyribonucleotidesFEBS Letters, 1973
- The corrected nucleotide sequence of yeast leucine transfer ribonucleic acidBiochemical and Biophysical Research Communications, 1973
- Primary sequence of glutamic acid tRNA II fromEscherichia coliFEBS Letters, 1972
- Nucleotide Sequence of the "Denaturable" Leucine Transfer RNA from YeastScience, 1971
- An evaluation of film detection methods for weak β-emitters, particularly tritiumAnalytical Biochemistry, 1970
- A two-dimensional fractionation procedure for radioactive nucleotidesJournal of Molecular Biology, 1965
- The detection of terminal nucleoside residues in polyribonucleotidesBiochimica et Biophysica Acta, 1960