Parameters and sequence-length distribution of enzymatic nucleotide copolycondensations

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Abstract
Primer-independent binary copolycondensations of all four common ribonucleoside diphosphates were done with catalysis by polynucleotide phosphorylase. The polymers obtained at low conversion were hydrolyzed, and the constituent ratio was determined by HPLC. The data were treated according to conventional copolymerization statistics, which allowed the calculation of copolymerization parameters for the 6 comonomer pairs. A good agreement was found between the sequence-length distribution as calculated from the r-parameters resp. determined from the degradation of poly (A,U) by ribonuclease A. Our results are evidence for a random incorporation of monomers into copolymers, however, no indication of a penultimate effect has been found.

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