Improvement of a Hemin Standard by Recrystallization

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Abstract
The purity of crude and of recrystallized hemin prepns. obtained from bovine and from human blood was detd. by analysis for Fe content by a micromodification of the titanous chloride method and by measuring the extinction coefficients at 580 m[mu]. Stock solns. were prepared by dissolving weighed amts. in 0.1 [image]borate buffer at pH 9.4; dilutions of these were allowed to stand for 1 mo. and the absorption spectra between 475-690 m[mu] were studied at intervals. The Fe content increased with recrystallization, becoming constant after the 3d repetition. Crude hemin prepns. in borate buffer showed variable extinction coefficients, but those of recrystallized hemin were nearly constant. Dilute soIns. of recrystallized hemins were more stable over a period of 1 mo. than the crude prepns. of the same concn. Stock solns. were stable regardless of purity.
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