Construction of a hybrid cDNA encoding a major legumin precursor polypeptide and its expression and localization in Saccharomyces cerevisiae
- 28 September 1987
- journal article
- Published by Wiley in FEBS Letters
- Vol. 222 (1) , 175-180
- https://doi.org/10.1016/0014-5793(87)80215-6
Abstract
A hybrid cDNA encoding a major legumin precursor polypeptide was constructed. Using a phosphoglycerate kinase expression vector levels of expression of 1–2% total protein were obtained in Saccharomyces cerevisiae. Immunocytochemical localization demonstrated that the legumin was accumulated within the Golgi vesicles.Keywords
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