Infusion and Translocation of Systemic Fungicides Applied to Seeds in Acetone

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Abstract
Systemic fungicides were applied to acid-delinted cottonseed [Gossypium hirsutum] and soybean [Glycine max (L.) Mers. cv. Amsoy] seed either directly (direct fungicide application) or by organic solvent (acetone) infusion. Seed coats of treated and untreated seeds were removed and the concentrations of the fungicides in seed coats and embryos (cotyledons + embryo) were estimated either indirectly by bioassay or directly by the use of radioisotopes. With either method of application, most of the fungicide remained in the seed coats. However, bioassays [using Rhizoctonia solani] revealed more fungicide within or upon the embryo in seeds treated with acetone infusion than in those that received it by direct application. The accumulation of fungicide in seeds exposed to acetone infusion increased as the seeds began to imbibe water. Fungicides in cotton and soybean seeds treated with acetone infusion were not leached out by water or acetone as readily as were those that had been applied by direct application. The translocation patterns and distributions of 14C-ethazol and 14C-carboxin were similar in cotton and soybean plants produced from seeds treated by either method.

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