Limitations of the Radioimmunoassay for Hepatitis B Antigen

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Abstract
To the Editor: Two hundred and forty-four laboratory personnel were screened for hepatitis B antigen by counterelectrophoresis, with the use of both human and guinea-pig antibody, by rheophoresis (Aus-Tect — Abbott Laboratories) and by radioimmunoassay (AusRIA — Abbott Laboratories). Both the latter tests use guinea-pig antibody.Twenty strong positive reactions (8.2 per cent) (Table 1) were found by radioimmunoassay, and three positive reactions (1.2 per cent) by counterelectrophoresis (in both human and guinea-pig systems). Two specimens were positive by rheophoresis (0.82 per cent), with lines of identity, and two gave strong precipitin lines, with no lines of identity. Those found . . .

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