EXTRACTION AND THIN-LAYER CHROMATOGRAPHY OF AFLATOXIN-B1 IN MIXED FEEDS

  • 1 January 1983
    • journal article
    • research article
    • Vol. 66  (3) , 582-586
Abstract
A method was developed for the determination of aflatoxin, B1 in commercially prepared feeds. The method incorporated methylene chloride and citric acid solution extraction, cleanup on a small silica gel column and TLC for quantitation. Commercial turkey starter, catfish chow, medicated pig starter, broiler finisher, rabbit chow, horse feed, rat chow and dog chow were investigated. The feeds were spiked with naturally contaminated corn at 4 different levels of aflatoxin B1 (16-130 .mu.g/kg). Three assays were run on each for the 32 combinations of feed and levels of aflatoxin. Mean recoveries were 85.9-92.8% at levels of 16.5, 32.9, 65.8 and 131.6 .mu.g/kg. The relative SD per assay was 18.6%. This method was more rapid and less involved than most previously published methods for mixed feeds.

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