Quantitative Analysis of Alkylacyl, Alkenylacyl, and Diacyl Types of Diglycerides Obtained from Glycerophospholipids
- 1 September 1987
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 10 (12) , 2799-2808
- https://doi.org/10.1080/01483918708066827
Abstract
The 1-alkyl-2-acyl, 1-alk-1'-enyl-2-acyl, and 1,2-diacyl-snglycerol moieties of choline and ethanolamine glycerophospholipids were converted to the dinitrobenzoyl derivatives. These compounds were separated by high performance liquid chromatography and quantitated by their absorbance at 235 nm. Peak areas were proportional to the amount injected. Separations were optimal for the derivatives from 5 to 30 nmol of the glycerophospholipids. The compositions of ethanolamine glycerophospholipids from rat brain and of choline and ethanolamine glycerophospholipids from human platelets agreed well with previous results obtained with much longer procedures.Keywords
This publication has 16 references indexed in Scilit:
- Separation of phospholipids by high-performance liquid chromatography: All major classes, including ethanolamine and choline plasmalogens, and most minor classes, including lysophosphatidylethanolamineJournal of Chromatography B: Biomedical Sciences and Applications, 1985
- A Sensitive Method for Quantitative Analysis of Phospholipid Molecular Species by High-Performance Liquid ChromatographyThe Journal of Biochemistry, 1985
- Chemical and biochemical aspects of platelet activating factor: A novel class of acetylated ether‐linked choline‐phospholipidsMedicinal Research Reviews, 1985
- Novel quantitative method for determination of molecular species of phospholipids and diglyceridesJournal of Chromatography A, 1984
- Chapter 2 Plasmalogens and O-alkyl glycerophospholipidsNew Comprehensive Biochemistry, 1982
- High-performance liquid chromatography of diglyceride p-nitrobenzoatesJournal of Chromatography A, 1980
- A method for the quantitative determination of glycerolipids containing O-alkyl and O-alk-1-enyl moietiesBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1975
- Preparation of Suspensions of Washed Platelets from HumansBritish Journal of Haematology, 1972
- Ethanolamine PlasmalogensPublished by Springer Nature ,1972
- Studies on the phospholipids of rat brain which contain glyceryl ethersBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1967