THE INCORPORATION OF P32-LABELED ORTHOPHOSPHATE AND GLYCEROL-1-3-C14 INTO THE LIPIDS OF THE POLYVINYL SPONGE GRANULOMA*

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Abstract
Inflammatory tissue induced by subcutaneous implantation of polyvinyl sponge will incorporate p32-orthophosphate and glycerol-1-3-C14 into phospholipids and neutral lipids in vivo and in vitro. Under the same experimental conditions, this capacity was found to be similar to that of liver. There was a definite difference observed in vitro between the labeling pattern of inflammatory tissue phospholipids after exposure to P32 and glycerol-C14 . With p32, major incorporation occurred in phosphatidyl choline, >monophosphoinositide > phosphatidyl ethanolamine > sphingo-myelin, whereas with glycerol-C14, monophosphoinositide and a component tentatively identified as phosphatidic acid were most highly labeled, > phosphatidyl choline and phosphatidyl ethanolamine. Approximately half of the glycerol-C14 incorporated into lipids was found in the neutral lipid fraction. The pattern of incorporation of both isotopes into liver phospholipids was found to be similar to that for glycerol-C14 incorporation by inflammatory tissue. In vitro incorporation of glycerol-Cl4 indicates that local de novo synthesis can contribute to the lipid content of the polyvinyl sponge granuloma.