Effects of Colchicine and Vinblastine on Axonal Transport of Choline Acetyltransferase in Rat Sciatic Nerve1

Abstract
The effects of colchicine (0.5-10-2 M) and vinblastine (10-2-10-5 M) upon axonal transport of choline acetyltransferase (CAT) and on nerve impulse conduction were investigated in the rat sciatic nerve. High concentrations of colchicine (0.5 M) and vinblastine (10-2 M) blocked completely both axonal transport of CAT and impulse conduction. Vinblastine (10-3 M) did not affect impulse conduction until 20-22 h after injection, but this concentration of vinblastine did block CAT transport; 5 .times. 10-5 and 10-4 M vinblastine blocked CAT transport but not impulse conduction. Colchicine (10-2 M and 10-1 M) were without effect on impulse conduction, but did produce substantial, although incomplete, block of CAT transport. Involvement of microtubules in transport of CAT was discussed.

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