Fluorometric determination of sisomicin, an aminoglycoside antibiotic, in dried blood spots on filter paper by reversed-phase high-performance liquid chromatography with pre-column derivatization.

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Abstract
A simple method for the determination of sisomicin (SISO) in dried blood spots (DBS) on filter paper has developed. SISO in DBS was recovered most effectively by ultrasonication in 0.05 M KH2PO4-borate buffer (pH 9.0). The eluates from the DBS were treated with a carboxymethyl-Sephadex column, followed by determination by reversed-phase high-performance liquid chromatography after pre-column fluorescence derivatization using O-phthalaldehyde and .beta.-mercaptopropionic acid in 0.05 M KH2PO4-borate buffer (pH 9.0). The detection limit of SISO was below 0.05 .mu.g per ml of whole blood (100 .mu.l). The method permits a simple blood collection method for monitoring of aminoglycoside antibiotics at therapeutic levels.

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