Localization of Tamm-Horsfall-glycoprotein-like immunoreactivity in cultured baby-hamster kidney cells, shown by immunofluorescence and by light- and electron-microscopic immunoperoxidase techniques

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Abstract
Tamm-Horsfall glycoprotein was isolated from hamster urine, and an antiserum against it was produced in rabbits. IgG was isolated from the antiserum. Immunocytochemical methods were used to localize Tamm-Horsfall-like immunoreactivity in 3 substrains of baby-hamster kidney (BHK) cells. Indirect immunofluorescence techniques showed that, in 2 substrains (BHK-21/C13/2P and BHK-21/C13/3P), a proportion of the cells fluoresced brilliantly, whereas those of the 3rd substrain (BHK-21/ICRF) were totally negative. Related findings were obtained by the immunoperoxidase optical-microscopic technique. From the results of immunoperoxidase techniques using the EM, it was concluded that the substance was present in association with the plasma membranes of the reacting cells. Data suggest that the line of baby-hamster kidney cells, BHK-21/C13, may contain cells of renal-tubular epithelial origin, and that a proportion of these may be variable from one subculture to another.

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