A CONTINUOUS FLUORIMETRIC ASSAY FOR ATPASE ACTIVITY
- 1 March 1990
- journal article
- research article
- Vol. 266 (2) , 611-614
Abstract
A new continuous coupled fluorimetric assay is described for ATPases in general. Thus phosphate released from ATP hydrolysis is coupled to the nucleoside phosphorylase reaction using 7-methylguanosine as a fluorescent substrate for the nucleoside phosphorylase reaction. The hydrolysis of 7-methylguanosine leads to 7-methylguanine, which has lower quantum yield and hence can be used to monitor ATP hydrolysis continuously. The method has the potential to be extended to GTPase and nucleotidyltransferase assays.This publication has 6 references indexed in Scilit:
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