Transformation ofStreptomyces avermitilis by plasmid DNA
- 1 October 1987
- journal article
- conference paper
- Published by Oxford University Press (OUP) in Journal of Industrial Microbiology & Biotechnology
- Vol. 2 (4) , 209-218
- https://doi.org/10.1007/bf01569542
Abstract
Polyethylene glycol (PEG) efficiently mediated the transformation ofStreptomyces avermitilis protoplasts by plasmid DNA to yield 107 transformants per μg of plasmid DNA. Under conditios in which the maximum transformation frequency was observed, the cotransformation frequency exceeded 10%. The number of transformants increased linearly with the amount of DNA and number ofS. avermitilis protoplasts. Relaxed and supercoiled, but not linear DNA transformed protoplasts efficiently. Dimethyl sulfoxide (DMSO)-mediated transformation of protoplasts was 1000-fold less efficient. PEG and, less efficiently, DMSO also mediated the transformation of whole cells ofS. avermitilis by DNA.This publication has 22 references indexed in Scilit:
- Characterization of the Streptomyces plasmid pVE1Plasmid, 1986
- A flexible boiling procedure for isolating plasmid DNA from gram-positive microorganismsJournal of Microbiological Methods, 1986
- Transfection and Transformation Systems for Pock-Forming and Thiostrepton-Producing Streptomyces azureusJournal of the Faculty of Agriculture, Kyushu University, 1985
- Isolation and characterization of the Streptomyces cattleya temperate phage TG1Gene, 1985
- New procedure for DNA transfection with polycation and dimethyl sulfoxide.Molecular and Cellular Biology, 1984
- pFJ265, a new cloning vehicle for StreptomycesPlasmid, 1984
- pIJ101, a multi-copy broad host-range Streptomyces plasmid: Functional analysis and development of DNA cloning vectorsMolecular Genetics and Genomics, 1982
- Avermectins, New Family of Potent Anthelmintic Agents: Producing Organism and FermentationAntimicrobial Agents and Chemotherapy, 1979
- Transformation of plasmid DNA into Streptomyces at high frequencyNature, 1978
- Transfection in Streptomyces virginiaeBiochemical Society Transactions, 1977