A cyclic AMP‐dependent phosphorylation of spectrin dimer
- 24 April 1984
- journal article
- Published by Wiley in FEBS Letters
- Vol. 169 (2) , 323-329
- https://doi.org/10.1016/0014-5793(84)80343-9
Abstract
In contrast to the properties of spectrin obtained from [32P]phosphate‐labeled red cells, purified spectrin dimer could be phosphorylated by a cAMP‐dependent protein kinase from bovine heart. Both spectrin bands were phosphorylated. Spectrin band 2 contained in addition to autophosphorylated peptides several phosphopeptides that were distinct from autophosphorylated ones. The cAMP‐dependent phosphorylation of spectrin band 1 was modulated by reducing agent and the concentration of spectrin. At high concentrations spectrin band 2 was predominantly labeled. The cAMP‐dependent phosphoform of spectrin band 2 had a pI slightly higher than that of autophosphorylated spectrin band 2, but lower than that of ankyrin.Keywords
This publication has 20 references indexed in Scilit:
- Glycoprotein topology on intact human red blood cells reevaluated by cross-linking following amino group supplementationBiochemistry, 1982
- In vitro phosphorylation of the red blood cell cytoskeleton complex by cyclic AMP-dependent protein kinase from erythrocyte membraneBiochimica et Biophysica Acta (BBA) - Biomembranes, 1981
- Interaction of cytoskeletal proteins on the human erythrocyte membraneCell, 1981
- Phosphorylation of intermediate filament proteins by cAMP-dependent protein kinasesCell, 1981
- Membrane Protein Phosphorylation and Protein Kinases in Normal and Hereditary Spherocytosis Red CellsHemoglobin, 1980
- The uptake of cyclic AMP by human erythrocytes and its effect on membrane phosphorylationArchives of Biochemistry and Biophysics, 1979
- Two-dimensional separation of erythrocyte membrane proteinsArchives of Biochemistry and Biophysics, 1979
- Self‐Association of Human SpectrinEuropean Journal of Biochemistry, 1978
- Vesicles isolated from ATP‐depleted erythrocytes and out of thrombocyte‐rich plasmaJournal of Supramolecular Structure, 1978
- Defective protein phosphorylation in membranes of hereditary spherocytosis erythrocytesFEBS Letters, 1974