STUDIES ON DELAYED-HYPERSENSITIVITY PLEURAL EXUDATES IN GUINEA-PIGS .1. DEMONSTRATION OF SUBSTANCES IN CELL-FREE EXUDATE WHICH CAUSE INHIBITION OF MONONUCLEAR CELL-MIGRATION INVITRO

  • 1 January 1976
    • journal article
    • research article
    • Vol. 30  (4) , 505-511
Abstract
Leukocyte migration inhibitory (MI) activity was investigated in vitro in the delayed hypersensitivity reaction induced by intrapleural injection of purified protein derivative into Freund''s complete adjuvant-sensitized guinea-pigs. During the initial reaction (6 h) 2 types of antigen-dependent MI activity were detected in serum and cell-free exudate. One was of high molecular weight and associated with immunoglobulin and the other was of low molecular weight and appeared to be related to antigen-dependent MI factor (F). As the reaction progressed (i.e., 12-24 h), 2 types of antigen-dependent MI activity were revealed in exudate, but not in serum. One was high molecular weight, and the other was low molecular weight and thought to be related to antigen-independent MIF. Similar experiments were performed on the reverse passive Arthus reaction in the pleural cavity of guinea-pigs. A high molecular weight MI activity was detected in 6-h cell-free exudate and was antigen-independent. MIF was not found in this reaction.