In vivo synthesis of complexN‐glycans by expression of humanN‐acetylglucosaminyltransferase I in the filamentous fungusTrichoderma reesei
Open Access
- 11 June 1999
- journal article
- Published by Wiley in FEBS Letters
- Vol. 452 (3) , 365-370
- https://doi.org/10.1016/s0014-5793(99)00648-1
Abstract
The humanN‐acetylglucosaminyltransferase I gene was introduced in the genome ofTrichoderma reeseistrain VTT‐D‐80133. Expression was studied after induction from the cellobiohydrolase I promoter. Successful in vivo transfer of GlcNAc was demonstrated by analyzing the neutralN‐glycans which were synthesized on cellobiohydrolase I. Final proof of the formation of GlcNAcMan5GlcNAc2was obtained by NMR analysis.Keywords
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