Prophage S2 Mutants in Haemophilus influenzae : A Technique for Their Production and Isolation
- 25 October 1968
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 162 (3852) , 464-465
- https://doi.org/10.1126/science.162.3852.464
Abstract
A procedure utilizing nitrosoguanidine has been developed to produce defective and temperature-sensitive mutants of prophage (S2) in lysogenic Haemophilus influenzae. The system should be generally applicable to all temperate phage systems. At saturating concentrations of phage DNA, more than 25 percent of recipient mutant lysogenic bacteria can be transformed to the wild type.Keywords
This publication has 4 references indexed in Scilit:
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- Hydroxylamine as a mutagenic and inactivating agentJournal of Molecular Biology, 1961
- The transformation of Escherichia coli with deoxyribonucleic acid isolated from bacteriophage λdgJournal of Molecular Biology, 1960
- The specific mutagenic effect of base analogues on Phage T4Journal of Molecular Biology, 1959