The Effect of Deuterium on the Release and Uptake of Noradrenaline in Isolated Nerve Granules

Abstract
The release of noradrenaline (NA) from isolated bovine splenic granules in phosphate buffer is decreased by 10–25 per cent by addition of 50–90 % D2O to the medium. The ac companying uptake of 14C(‐)NA is unchanged in 50–70 % D2O and slgihtly decreased in 95 % D2O. After previous partial NA depletion of the granules the net uptake of NA in the presence of ATP‐Mg2+ is lowered by D2O in concentrations above 25 % in the medium and has fallen to 63 per cent of normal with 87.5 % D2O.