In vitro AND in vivo UPTAKE OF BENZOPORPHYRIN DERIVATIVE INTO HUMAN AND MINISWINE ATHEROSCLEROTIC PLAQUE

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Abstract
Abstract— Befnzoporphyrin derivative(BPD) has been demonstrated to be fnew potent photosentsitze for photodynamic therapy(PDT). Althought most of wrok on BPD has been focused on its potential applications for cancer tratment, BPD amy have potential clilnical uses in the treatment of artheros clerosis. The purposes of this study was to determine in vitro and vivo uptake of BPD into atherosclerotic plaque. Samples of atherosclerotic human femoral and popliteal arteries were incubated with BPD‐monoacid, ring A(BPD‐MA) for 1 h in the following concentrations: 1, 5, 10, 20, 30 and 40 μg/mL. fluorescence from all samplesd was determined by chemical etraction with a spectrofluorometer. the tissue concentration for human arteries was 0.37 ± 0.03, 2.78 ± 1.5, 3.6 ± 1.91, 7.15 ± 2.36, 8.06 ± 3.09 and 14.6 ± 4.81 μg/g, respectively. In aeddition, three miniswine were rendered atherosclerotic and given BPD 2.0 mg/ Kg intravenously. The concentration of BPD‐MA in miniswine aorta was93–190 ng/g and the plaque/normal ration was 1.7–3.5, for miniswine cartoid artery contained 54 ng/g. this study showed that BPD‐MA was taken up in atherosclerotic vesselsd both in vitro and in vivo and mey have potential for PDT of atherosclerosis.