Comparison of kinetic and end-point diffusion methods for quantitating human serum immunoglobulins

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Abstract
Proficiency testing results were used to compare the kinetic and end-point versions of the single radial immunodiffusion method for quantitating human serum immunoglobulins. Statistical analysis of the results indicated that the results were not normally distributed but that the log normal distribution gives the best fit of any of the well-known frequency distributions. Consequently, statistical analysis of immunoglobulin results must be log transformed before parametric statistical tests can be appropriately applied. In general, there were no significant differences in level, precision, or interlaboratory comparability for these two methods. However, levels were different for participants using Hyland reagents, and better interlaboratory comparability was achieved by the end-point assay for immunoglobulin M. There were no significant differences in reported levels when compared by manufacturer within the same method.