Nicotinamide Adenine Dinucleotide-dependent Proline Dehydrogenase in Chlorella
Open Access
- 1 March 1974
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 53 (3) , 440-444
- https://doi.org/10.1104/pp.53.3.440
Abstract
An NAD-linked dehydrogenase from Chlorella pyrenoidosa Chick catalyzing the conversion of l-proline to Δ1-pyrroline-5-carboxylic acid was partially purified. Δ1-Pyrroline-5-carboxylic acid was identified as the product by co-chromatography of it and its o-aminobenzaldehyde derivative with authentic compounds. The enzyme is NAD and l-proline specific and is not an oxidase; NADP is not inhibitory. The Michaelis constant for NAD is 0.08 mm and for proline is 0.73 mm.Keywords
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