Blood Group Antibody Activity among γ1A-Globulins

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Abstract
Summary: Evidence is presented that γ1A-globulins carry specific blood group antibody activity. In Coombs-like reactions anti-γ1A uniformly agglutinated anti-D and anti-G sensitized RBC but only rarely agglutinated cells sensitized by antibodies to other blood group factors. When found in these other blood group systems, the agglutination reactions were weak. Addition of purified γ1A-globulin to anti-γ1A-globulin and to anti-γ-globulin reduced the ability of the former to agglutinate anti-D sensitized red cells but was without effect on anti-γ-globulin reactions. Fractionation of various antisera by DEAE-cellulose chromatography and by sucrose density gradient centrifugation led to identification of the γ1A antigen only in those antibodies in which it was detected in studies with whole serum. Presumptive evidence is presented that γ1A-globulins in various states of polymerization may function as antibodies.

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