EFFECT OF BURST-PROMOTING ACTIVITY (BPA) AND ERYTHROPOIETIN ON HEMOGLOBIN BIOSYNTHESIS IN CULTURE
- 1 January 1980
- journal article
- research article
- Vol. 56 (6) , 1106-1110
Abstract
The influence of erythropoietin (Ep) and burst-promoting activity (BPA) on the relative rates of synthesis of fetal and adult Hb (HbF and HbA) were investigated on clonal human cell cultures. In the case of crude Ep, a positive dose-response relationship between the concentration and the relative rate of HbF biosynthesis by circulating erythropoietic precursors was clearly observed. Increasing the concentration of purified Ep enhanced the synthesis of HbF and HbA equally. BPA from bone marrow conditioned media and T-cell conditioned media enhanced the relative rate of HbF biosynthesis and burst number at limiting serum concentrations (10%-15%). BPA is important in the modulation of HbF biosynthesis in culture.This publication has 12 references indexed in Scilit:
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