Identification of microtubule-associated proteins in the meiotic spindle of surf clam oocytes.

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Abstract
Meiotic spindles isolated from surf clam oocytes to morphological purity are biochemically complex, consisting of many polypeptides. These proteins fall into 2 classes: polypeptides that are apparently cytoplasmic proteins and are not specifically associated with the spindle, and polypeptides that are specifically associated with the spindle. A subset of the spindle-associated proteins, including a 250,000 MW component, remain with spindle tubulin through cycles of cold depolymerization and warm polymerization, showing that they are microtubule-associated proteins.