Dominant-Negative Suppression of HCN Channels Markedly Reduces the Native Pacemaker Current I f and Undermines Spontaneous Beating of Neonatal Cardiomyocytes

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Abstract
Background— The pacemaker current I f contributes to spontaneous diastolic depolarization of cardiac autonomic cells. In heterologous expression, HCN channels exhibit a hyperpolarization-activated inward current similar to I f . However, the links between HCN genes and native I f are largely inferential, and it remains unknown whether I f is essential for cardiac pacing. Methods and Results— To clarify this situation, we generated a GYG 402–404 AYA pore mutation of HCN2, which rendered the channel nonfunctional and suppressed wild-type HCN2 in a dominant-negative manner in Chinese hamster ovary cells. In addition, HCN2-AYA suppressed I HCN4 in a dominant-negative manner when coexpressed with wild-type HCN4, indicating that the 2 isoforms HCN2 and HCN4 are able to coassemble to form heteromultimeric complexes. Given that HCN2 and HCN4 are the dominant HCN mRNA transcripts in neonatal rat ventricle, we expressed HCN2-AYA in neonatal cardiocytes using adenoviral gene transfer to test the effect of HCN suppression on native I f . I f density was indeed reduced markedly, from 7.8±1.6 pA/pF (n=13) in control cells to 0.3±0.2 pA/pF (n=11) in HCN2-AYA–infected cells when measured at −130 mV ( P P Conclusions— HCN channels are the major molecular component of native I f and are critical for spontaneous beating of neonatal cardiomyocytes.